FastQCFastQC Report
Tue 10 Dec 2019
HYCT7AFXY_n02_ncm6-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCT7AFXY_n02_ncm6-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9153495
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG147190.1608019668989823No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGATC35350.028.0246775
CCGTATC244300.027.06404755
CGTATCA250950.026.73740256
CGCCGTA257050.026.64756453
TCGCCGT259500.026.28807452
GTCGCCG274300.026.08190751
GCCGTAT271700.026.07384954
GGTCGCC276550.025.54064250
TGGTCGC276350.024.9765149
GTATCAT254750.024.77200157
CTCGGTG249650.024.52017244
GTGGTCG287350.024.31285748
TCTCGGT254050.024.15073643
ATCTCGG261100.023.8725442
TCATTAA278750.023.60497360
GATCTCG255900.023.56389241
CATTAAA286650.023.5524561
GGTGGTC295450.023.3547
TCGGTGG294150.022.88166445
ATCATTA288450.022.61704859
Produced by FastQC (version 0.11.5)