FastQCFastQC Report
Tue 10 Dec 2019
HYCT7AFXY_n02_ncm6-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCT7AFXY_n02_ncm6-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14924275
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG237380.1590563025674614No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA213500.024.65639153
CCGTATC210200.024.39383755
TCGCCGT215650.024.15088552
GGTCGCC239300.024.01667850
GTCGCCG239050.023.9246651
GCCGTAT236750.023.44721854
CGTATCA226400.023.3134256
TGGTCGC234050.023.0898549
CTCGGTG232450.022.40451244
ATCTCGG243300.022.18092342
TCTCGGT237250.021.96583443
GTGGTCG256300.021.8637848
GATCTCG243150.021.84909241
AGATCTC258500.020.6189540
GGTGGTC272300.020.52705647
GTATCAT247850.020.36359457
TCGGTGG284750.020.24413745
CGAGATC45050.019.5815225
TAGATCT289400.019.30012139
CGGTGGT305700.018.68504146
Produced by FastQC (version 0.11.5)