FastQCFastQC Report
Tue 10 Dec 2019
HYCT7AFXY_n02_ncm6-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCT7AFXY_n02_ncm6-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16735288
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG265700.158766314628108No Hit
CGGGAAGGAATAAGAAGCAACTAAACGAGGGTGTAGAAAAAGACGAAGAG190180.11364011184032208No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC322800.026.7396555
CGCCGTA336400.026.5848253
CGTATCA335650.026.0705956
TCGCCGT341950.025.87695952
GCCGTAT359850.025.84461854
GTCGCCG358750.025.7772851
GGTCGCC362000.025.51677150
TGGTCGC365200.024.74657849
CTCGGTG339900.024.09544244
GTGGTCG383500.023.91253548
GTATCAT351150.023.80305357
TCTCGGT345650.023.67407243
GATCTCG347250.023.23147841
ATCTCGG356950.023.2081442
GGTGGTC401200.022.7350947
TCGGTGG403350.022.24891345
AGATCTC374750.021.83345440
TCATTAA397900.021.83196860
CGAGATC56050.021.4851685
CATTAAA416350.021.37691561
Produced by FastQC (version 0.11.5)