FastQCFastQC Report
Tue 10 Dec 2019
HYCT7AFXY_n02_ncm6-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCT7AFXY_n02_ncm6-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7237642
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG138670.1915955500424033No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGATC25900.032.7083365
CCGTATC246250.024.54668655
CGTATCA250400.024.44754656
CGCCGTA263550.023.51989453
GCCGTAT278850.023.1204254
TCGCCGT265750.023.04844352
CATTAAA266700.022.82087961
GTCGCCG282250.022.58165651
TGTCGGC18000.022.55273630
GTATCAT259550.022.41231757
GGTCGCC287250.022.20077550
TCATTAA268850.022.17003660
AAAAGGG123850.021.8437570
GCGAGAT15550.021.8412384
TGGTCGC290550.021.67140649
CTCGGTG262300.021.6161644
CTAGATC24150.021.5979215
ATCTCGG267000.021.44493942
TCTCGGT264850.021.43461443
GATCTCG257850.021.43225141
Produced by FastQC (version 0.11.5)