FastQCFastQC Report
Tue 10 Dec 2019
HYCT7AFXY_n02_ncm6-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCT7AFXY_n02_ncm6-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4464567
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG81400.1823245120971418No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGATC15200.032.2433555
CTAGATC16650.025.230325
CCGTATC133000.024.63214155
CGTATCA135950.024.40658656
CGCCGTA141150.024.3758853
TCGCCGT141100.023.86358652
GCCGTAT152900.023.53251854
GTCGCCG161350.023.05960851
GGTCGCC162350.022.87394150
TGGTCGC157000.022.58356949
AAAGGGG45550.022.12804670
CTCGGTG153500.022.07092544
GTATCAT146300.021.91412757
CATTAAA157900.021.85438761
ATCTCGG157000.021.80084442
TCTCGGT154450.021.75364543
GTGGTCG168750.021.5503648
AAGATCG82350.021.5494846
GATCTCG156500.021.49009541
CGCGCAG2301.2732926E-1121.3373451
Produced by FastQC (version 0.11.5)