FastQCFastQC Report
Tue 10 Dec 2019
HYCT7AFXY_n02_ncm6-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCT7AFXY_n02_ncm6-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7915922
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG145100.18330145243977897No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGATC20600.030.758595
CCGTATC167450.022.84717255
CGCGAAG9400.022.7529831
CGCCGTA176200.022.74543853
CGTATCA172300.022.69161256
TCGCCGT175950.022.4395752
TGTCGGC15700.022.06667930
GTCGCCG197000.022.04957651
GGTCGCC199000.021.89846850
CGGCGGC16000.021.653233
GCCGTAT195200.021.58957554
TGGTCGC195100.021.42096349
CTCGGTG188150.021.18757644
CGCGAAA11450.021.1290511
TCTCGGT190550.020.88384843
ATCTCGG195200.020.60034842
GATCTCG191150.020.5424841
GTGGTCG211200.020.36794348
CGCGCAG3800.020.2990381
AGATCTC196600.020.0078640
Produced by FastQC (version 0.11.5)