FastQCFastQC Report
Tue 10 Dec 2019
HYCT7AFXY_n02_ncm6-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCT7AFXY_n02_ncm6-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8759311
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG149430.17059560963185344No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCC3300.025.45358766
CGGGCAG7300.024.0056951
CGAGATC23400.022.8881955
CGCGAAG10500.022.364161
CGGCAAG19250.020.5738161
GCGAGCG13550.020.14569311
GGCGAGC14400.019.92832610
CGCGCAG4250.018.9673231
CGTATCA194550.018.96207456
CCGTATC191600.018.8521455
CGCCAAA27250.018.6495631
CGAGCAG14100.018.3941521
CGCCGTA206350.018.20001453
CGCGTAG4050.018.165722
TGTCGGC16100.018.04135530
TCGCCGT206600.017.94081352
GCCGTAT222900.017.38257254
GTCCGCA25100.017.28949513
CGGCAAT20750.017.2285671
CGAGCGT16300.017.17667812
Produced by FastQC (version 0.11.5)