FastQCFastQC Report
Tue 10 Dec 2019
HYCT7AFXY_n02_ncm6-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCT7AFXY_n02_ncm6-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8077875
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG128730.15936121814214754No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGATC29800.023.0242615
CGCGAAA16150.021.7091921
CGCGAAG12450.020.2758641
CGGCAAG21300.020.0815121
CGTATCA239400.018.97801256
CCGTATC236550.018.94031555
CGCCGTA252350.018.53130553
CGCGGAG7200.018.5040721
GTATCAT238600.018.08798257
CGGGAAG44600.018.0804461
TCGCCGT256350.018.07829952
GCCGTAT269750.018.03666554
CGCGTAG3500.018.0229852
CATTAAA259550.017.97489261
CGGCAAA50600.017.945911
CGAGCAG15100.017.8784541
CGGGCAG8850.017.8272931
CTAGATC31100.017.6719785
GTCGCCG274150.017.51757851
TCATTAA260400.017.47300360
Produced by FastQC (version 0.11.5)