FastQCFastQC Report
Tue 10 Dec 2019
HYCT7AFXY_n02_ncm6-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCT7AFXY_n02_ncm6-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7193380
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG119570.16622227659320096No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA76250.022.12487653
TCGCCGT76100.022.03050852
CCGTATC76700.021.40154355
GTCGCCG99850.021.24213851
GGTCGCC99000.020.92941950
CGGCAAG19700.020.638251
GCCGTAT91800.020.28334654
CTCGGTG105650.020.20793344
CGTATCA87650.020.16541156
TGGTCGC91150.020.1593449
ATCTCGG103250.019.89745542
TCTCGGT103450.019.8595443
GATCTCG104550.019.41558341
GTGGTCG112250.019.14501448
GGTGGTC119150.017.97732447
AGATCTC106850.017.78536240
TCGGTGG144950.017.62638345
CGAGATC22450.017.6192365
CGGGAAG38550.017.5474511
CGCGAAA14650.017.4649091
Produced by FastQC (version 0.11.5)