FastQCFastQC Report
Tue 10 Dec 2019
HYCT7AFXY_n02_ncm6-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCT7AFXY_n02_ncm6-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7944200
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG119150.14998363586012436No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGATC32350.028.2415775
CCGTATC258400.023.92148255
CGTATCA265400.023.69938356
CGCCGTA271700.023.48495153
TCGCCGT273250.023.22363952
GCCGTAT290550.022.97339454
GTATCAT264350.022.72087757
GTCGCCG297950.022.67287351
CATTAAA289350.022.6183861
TCATTAA282700.022.51948560
GGTCGCC300350.022.50335550
TGGTCGC297050.022.25859649
CGGCAAG22350.022.1159041
CTCGGTG273300.021.91195544
TCTCGGT277300.021.62071443
GTGGTCG313350.021.51390348
GATCTCG276750.021.42231641
ATCTCGG285200.021.41344642
GGTGGTC318250.021.14926547
ATCATTA300100.021.02719359
Produced by FastQC (version 0.11.5)