FastQCFastQC Report
Tue 10 Dec 2019
HYCT7AFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCT7AFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11377992
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6459385.677082564304844No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT56050.022.5286371
AAGATCG126400.021.9952016
GAGATCG84450.021.9888616
CACTCTT90250.020.9678251
CGTCTGA291050.018.11646723
ACGTCTG291750.018.08381522
CACACGT298650.017.98583619
AAAGATC56650.017.9629675
CAGTCAC245000.017.9536934
GAACTCC277750.017.87186428
GCACACG303450.017.82418618
ACACGTC298600.017.8014220
CCAGTCA250850.017.79991133
CACGTCT298150.017.778921
TGAACTC288050.017.66004827
CTGAACT288300.017.6326626
AGCACAC308500.017.59952717
AGAGCAC316300.017.59570915
GCGAGAT16350.017.5380424
GATCGGA331950.017.3703338
Produced by FastQC (version 0.11.5)