FastQCFastQC Report
Tue 10 Dec 2019
HYCT7AFXY_n01_ncm6-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCT7AFXY_n01_ncm6-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11590711
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT66400.026.89061
CTGCTTG159800.022.86584564
GCTTGAA174200.022.52262966
TGCTTGA173600.021.14897365
GCCGTCT216700.019.88254756
CACTCTT137000.019.3963321
CCGTCTT232050.018.7784957
TGCCGTC251750.018.64370355
ATGCCGT264550.017.91363754
TATGCCG270500.017.67464453
TCTGCTT214800.017.36946363
GAGATCG97450.017.0252596
GTATGCC280250.016.98480252
CTTGAAA238600.016.94233167
CGTATGC284450.016.8202251
CTTCTGC239800.016.36138261
GATCTCG268600.016.18367846
TCGTATG292450.016.0848450
CTCGTAT281450.016.07927349
CGTCTTC274100.015.83381458
Produced by FastQC (version 0.11.5)