FastQCFastQC Report
Tue 10 Dec 2019
HYCT7AFXY_n01_ncm6-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCT7AFXY_n01_ncm6-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13769959
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT73450.023.7361741
ATCGGGG205550.022.93583147
GAGATCG160150.022.7514276
AATCGGG247900.022.64603446
TGGCGGG62700.022.49614755
GGGTGGA40650.022.47205266
AAGATCG225150.021.8729326
GAATCGG262200.021.83810445
CGGGGTT90750.021.52055549
GCGGGGT69200.021.242957
AATCGCG78800.021.14195346
TGAATCG358100.021.09169444
TCGGGGT100500.021.03464748
GTCACAC499950.020.9879236
ACACTTG506700.020.52873839
AGTCACA511350.020.4789535
CGGCGTG32900.020.42565558
CACTTGA508850.020.41441240
TCACACT517750.020.34748637
CACACTT518550.020.2418538
Produced by FastQC (version 0.11.5)