FastQCFastQC Report
Tue 10 Dec 2019
HYCT7AFXY_n01_ncm6-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCT7AFXY_n01_ncm6-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9153495
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT54100.029.5732481
GCCGGCG19150.025.58805756
CCGGCGT21350.024.42679457
ATCGGGG83550.023.75188447
GGGTGGA31450.023.5925266
CATCGGG113450.023.44609846
TGCCGGC28050.023.08431455
TGCCGGG30400.022.5663155
AAGATCG182250.022.394186
GAGATCG121050.022.2942946
GCGTGGG21350.022.13177960
GCGTGAA25200.021.52733466
GATCATC301300.020.9555443
CAGATCA386500.020.85479541
TGGGGGT52050.020.84502663
AGTCACC412200.020.52249335
CGGGGTT50050.020.489249
GGGCGTG17600.020.48264564
CAGTCAC414950.020.43709434
CCAGTCA416900.020.3749733
Produced by FastQC (version 0.11.5)