FastQCFastQC Report
Tue 10 Dec 2019
HYCT7AFXY_n01_ncm6-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCT7AFXY_n01_ncm6-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14924275
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT71400.024.9092671
GCGTGAA59050.024.30099766
GCCGTCT174900.023.33337456
TGCCGTC225400.022.62418755
TATGCCG252050.022.21740753
ATGCCGT239300.021.7341954
GTATGCC264700.021.45982252
GCTTGAA170050.021.21984366
CCGTCTT201800.020.7433557
CGTATGC277000.020.22894151
CTGCTTG146100.020.1947764
TGCGTGA45400.020.12084665
GAGATCG125800.020.0339156
TATCTCG316650.019.82910246
CTCGTAT278300.019.61882449
GTCACGC387400.019.60470236
ATCTCGT311450.019.48591847
TCACGCC392600.019.31829337
AGTCACG394850.019.30571435
TGCTTGA159350.019.26234265
Produced by FastQC (version 0.11.5)