FastQCFastQC Report
Tue 10 Dec 2019
HYCT7AFXY_n01_ncm6-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCT7AFXY_n01_ncm6-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16735288
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT84950.024.1916791
GCCGGCG41800.023.78025856
GCGTGAA56800.023.23021766
GAGATCG169450.022.5156966
TGCCGGG40150.022.40388755
CCGGCGT49250.022.24398657
TGCCGGC63200.021.8753955
ATGCCGG70050.021.18524754
GCGGGGT44800.021.09406357
CACAGTG534000.020.80305940
GTCACAC539700.020.6223236
TATGCCG99100.020.59012853
AAGATCG256050.020.4096476
GGGTGGA42650.020.35133466
ACACAGT551700.020.13576339
GATCGCG35900.020.08326546
CAGTGAT503850.020.00565342
AGTCACA560400.019.87306635
CACACAG561300.019.8412638
GTCGGGG16900.019.67426162
Produced by FastQC (version 0.11.5)