FastQCFastQC Report
Tue 10 Dec 2019
HYCT7AFXY_n01_ncm6-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCT7AFXY_n01_ncm6-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7237642
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT52850.041.4652861
GCTTGAA149000.029.83182166
CTGCTTG138450.028.060364
GCGTGAA37700.027.8510966
TGCTTGA145650.027.37004565
GGGATTC26150.025.02834932
CGTGAAA44000.024.81785267
TGCGTGA28600.024.59757865
CTTGAAA189750.024.32905867
GAAAAAA274250.024.05619870
CTGCGTG29000.023.89623664
TGAAAAA262000.023.67144469
GGCGTGA14500.023.41348365
GCCGTCT208400.023.32774756
TCTGCTT172500.023.17070863
CCGTCTT219750.022.9192457
GAGATCG111250.022.4644876
TGCCGTC257000.022.30740555
ATGCCGT269400.021.73535254
TGTTGGG32000.021.54658928
Produced by FastQC (version 0.11.5)