FastQCFastQC Report
Tue 10 Dec 2019
HYCT7AFXY_n01_ncm6-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCT7AFXY_n01_ncm6-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4464567
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT30250.044.0911181
GCGTGAA16600.030.782566
CGTGAAA20550.026.73910967
GGCGTGA9200.026.63018265
GAGATCG68950.025.2307326
GGGTGGA13450.024.72067866
GCCGGCG12200.024.3847456
GGGTGTA12200.024.09786266
CCGGCGT13250.024.03724157
GGGCGTG8800.023.46568964
TGCGTGA11650.023.43325665
CACTCTT73850.023.4168221
CTGCGTG9350.023.20833664
GCCGTCT15600.022.88414456
GCGTGGA6450.022.79022866
GCGAGAT11750.022.6403664
GGAGATC14200.022.4316275
CGTGGGC5150.021.7473761
CGCGAGA5050.021.4871123
CGGGGTG29800.021.2579558
Produced by FastQC (version 0.11.5)