FastQCFastQC Report
Tue 10 Dec 2019
HYCT7AFXY_n01_ncm6-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCT7AFXY_n01_ncm6-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7915922
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT44400.039.268471
GCGTGAA24600.025.46703766
CCGGCGT21150.023.49870557
GCCGGCG20250.023.33322156
GAGATCG84850.022.689426
TGCCGGC29750.022.1175455
CGTGAAA32600.021.04257867
GGCGTGA11850.020.9702365
TGCGTGA18800.020.66466565
CACTCTT109100.020.37731
GGGTGGA18150.020.05474766
GGGCGTG13550.019.88907864
ATGCCGG32150.019.81328254
AAGATCG137450.019.7874076
CTGCGTG16450.019.78701464
TGCCGGG21250.019.59990755
GGGTGTA18300.019.31660566
GTGCGTG14050.019.18128264
GTGGGCG13100.018.96925562
GCGTGGA11450.018.95163566
Produced by FastQC (version 0.11.5)