FastQCFastQC Report
Tue 10 Dec 2019
HYCT7AFXY_n01_ncm6-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCT7AFXY_n01_ncm6-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8759311
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT59350.039.285161
GCTTGAA129650.025.15963666
CACTCTT137000.023.4072251
CTGCTTG126700.022.7623264
TGCTTGA132300.022.46020765
GCGTGAA26900.022.24870766
CGGCGTG11650.020.42908564
GGCGTGA11850.019.78893365
GTGGCGG12600.019.72212640
CTTGAAA172150.019.68019167
AGTGGCG13800.019.02164639
GGCGGTA13350.018.87631242
TCTGCTT161500.018.39929263
GCCGTCT199950.018.09943856
GCGGTAG13550.017.82279243
CCGTCTT210600.017.56639157
CGTGAAA37150.017.42905667
TGAAAAA254450.017.23493269
GGTTGCC13150.017.03394168
TGCCGTC240400.016.87387755
Produced by FastQC (version 0.11.5)