FastQCFastQC Report
Tue 10 Dec 2019
HYCT7AFXY_n01_ncm6-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCT7AFXY_n01_ncm6-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8077875
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT60750.038.671521
CACTCTT120950.022.4921071
GCGTGAA43100.021.60061566
CGTGAAA52150.019.52991967
GTCGGGG7450.018.32188262
GGCGTGA18550.018.3016265
TGCGTGA33600.016.9789265
GTGCGTG15500.016.70943564
TGCCGTC98750.016.4810855
GCCGTCT66650.016.43667656
TATGCCG127050.016.363453
AAGATCG181700.016.0088066
CTGCGTG28900.015.98592964
TCGGCGT22400.015.93726863
TGCCGGC38050.015.91333755
ATGCCGT106750.015.770555554
CCGGGGT19550.015.7545457
GCGGGGT21950.015.62648757
GGGTGGA19300.015.59562866
GAGATCG124350.015.5665426
Produced by FastQC (version 0.11.5)