FastQCFastQC Report
Tue 10 Dec 2019
HYCT7AFXY_n01_ncm6-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCT7AFXY_n01_ncm6-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7193380
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT44850.033.8752251
GCGTGAA31200.022.43570566
CTGCTTG84650.022.12030864
GCTTGAA99700.021.65979466
GCCGTCT123650.020.57849756
TGCTTGA94850.020.2950365
CACTCTT90700.020.1859551
CTGCGTG23050.019.73952764
CCGTCTT135950.019.28306457
TGCCGTC158000.019.25023855
ATGCCGT168100.018.48922254
TATGCCG178400.018.46129453
TGCGTGA25950.018.20793765
GTATGCC187000.018.04275352
CGTATGC189500.017.78625151
CGTGAAA42600.017.74632667
GAGATCG78850.017.3118046
CGGCGTG12550.017.2906964
GGCGTGA12000.017.20818565
TCGTATG194000.016.5798750
Produced by FastQC (version 0.11.5)