FastQCFastQC Report
Tue 10 Dec 2019
HYCT7AFXY_n01_ncm6-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCT7AFXY_n01_ncm6-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7944200
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT59800.036.9986761
CACTCTT123050.025.2639711
GGGTGTA25850.022.6108566
GCGTGAA23250.022.58027666
GGGTGGA29500.022.4233666
GAGATCG122750.021.9858386
CCGGCGT24050.021.3933957
CCGGGGT25600.021.1918657
GCGTGGG20000.021.0004560
GGGGGGA24950.020.76118366
TGGGGGT44250.020.40644663
AAGATCG186850.020.1384266
TGCCGGG29800.020.08432455
TGCCGGC30150.019.9672655
GCCGGCG21900.019.65795756
CGGGGTG59650.019.36336558
CGGCGTG31500.019.33374858
GCCGGGG24550.019.24684756
GGGGGAA28050.019.21537267
CGGGGTT66900.018.8860949
Produced by FastQC (version 0.11.5)