FastQCFastQC Report
Mon 5 Apr 2021
HYCCVBGXG_n01_JK26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCCVBGXG_n01_JK26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14829555
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC3579482.4137474118407463TruSeq Adapter, Index 2 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTATGC1532111.033146308166361TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGGATGC487740.3288972595603846TruSeq Adapter, Index 2 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATTC422260.2847421921965966TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTTTGC402550.27145116626898114TruSeq Adapter, Index 2 (97% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTTTGC393940.26564519299466505TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTATGG329770.22237349670977988TruSeq Adapter, Index 2 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGGTTGG303010.20432845085371745TruSeq Adapter, Index 2 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGG285290.19237933977115296TruSeq Adapter, Index 2 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTATTC274360.1850089230593905TruSeq Adapter, Index 2 (97% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGGATGC273840.1846582719441008TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGTATGC255620.1723719963276039TruSeq Adapter, Index 2 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGGTTGC207780.14011209372095115TruSeq Adapter, Index 2 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGGATGG204070.1376103328791727TruSeq Adapter, Index 2 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGTTTGG196880.1327619068812247TruSeq Adapter, Index 2 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTTTGG194890.13141999203617372TruSeq Adapter, Index 2 (97% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTGGTATGC190510.12846643071892583TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGGATGC190030.12814275276635073TruSeq Adapter, Index 2 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGTTTGC175680.1184661306424906TruSeq Adapter, Index 2 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGGATGG175140.11810199294584361TruSeq Adapter, Index 2 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGGTTGC168310.11349632541232695TruSeq Adapter, Index 2 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGGTTGG159840.10778475820751195TruSeq Adapter, Index 2 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGGGTGG159680.10767686555665358TruSeq Adapter, Index 2 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGTATGG159670.10767012226597494TruSeq Adapter, Index 2 (97% over 42bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)