FastQCFastQC Report
Mon 5 Apr 2021
HYCCVBGXG_n01_JK25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCCVBGXG_n01_JK25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13035434
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC8572126.576014270027374TruSeq Adapter, Index 1 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATATCGTATGC149060.1143498559388203TruSeq Adapter, Index 1 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATGTCGTATGC142820.10956290369772115TruSeq Adapter, Index 1 (98% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG134900.10348715662247991No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAGCTCGTATGC132190.1014082078126436TruSeq Adapter, Index 1 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)