FastQCFastQC Report
Mon 5 Apr 2021
HYCCVBGXG_n01_AKM211.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYCCVBGXG_n01_AKM211.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13049705
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT1413241.0829670095990676TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGCGTAT273230.20937638053887042TruSeq Adapter, Index 14 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTTT170030.13029413308576707TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)