FastQCFastQC Report
Thu 19 Jul 2018
HY7YMBGX5_n01_dam_gr_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHY7YMBGX5_n01_dam_gr_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45118740
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGA2681840.5943960314494597No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGA1735930.3847470031299633No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGATC8865950.063.4130811
AGGATCC2135000.063.22543312
AGGATCG731950.063.10065512
AGGATCA2981600.062.99148612
AGGATCT3075200.062.91445512
GGATCCA899750.062.80375313
CGAGGAT8995250.062.6102510
GGATCTC776400.062.51756713
GGATCCC344150.062.3790113
GGATCAC479250.062.37191813
GGATCAT1084050.062.30895213
GGATCGT202150.061.99733713
GGATCTA591050.061.8836813
GGATCTG525550.061.68507413
GGATCAA1065950.061.5053613
GGATCGC179300.061.2317513
GGATCAG408950.061.0784413
GGATCTT1245350.061.06478513
GGATCCG320050.060.7994213
CGGCCGA9348550.060.5976036
Produced by FastQC (version 0.11.2)