FastQCFastQC Report
Thu 19 Jul 2018
HY7YMBGX5_n01_dam_gr_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHY7YMBGX5_n01_dam_gr_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40543584
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGA625960.1543918761597396No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGA439150.10831553520280791No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGATC12507900.064.6126111
AGGATCG798150.064.5275812
AGGATCT4469850.064.5209512
AGGATCA4336150.064.3949312
AGGATCC2942950.064.35390512
GGATCTC1154450.064.2175313
GGATCTT1756450.064.1695113
GGATCCA1280550.064.16611513
CGAGGAT12611100.064.1384310
GGATCTA852250.064.1028213
GGATCCC446500.064.0307613
GGATCCG349250.064.0232513
GGATCAT1646450.063.9481913
GGATCTG737550.063.83172613
CGGCCGA12710900.063.8236776
GGATCAA1532550.063.8138813
GCGGCCG12729750.063.7241865
CGCGGCC12730800.063.6677974
GGTCGCG12690150.063.6593441
CCGAGGA12735150.063.6106769
Produced by FastQC (version 0.11.2)