FastQCFastQC Report
Thu 19 Jul 2018
HY7YMBGX5_n01_dam_gr_bzip1_r3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHY7YMBGX5_n01_dam_gr_bzip1_r3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28410188
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGA343480.1209002911209176No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGATC9076950.065.1621411
AGGATCT3259250.064.82204412
AGGATCA2949550.064.7676912
AGGATCC2166550.064.7165912
CGAGGAT9155350.064.70849610
AGGATCG765200.064.3608412
CGGCCGA9281650.064.26856
GGATCCA927000.064.2648213
GGATCTC774800.064.2185813
GCGGCCG9303900.064.106265
CCGAGGA9274850.064.097519
GGCCGAG9307750.064.085167
GCCGAGG9307950.064.084618
CGCGGCC9299650.064.0681844
GGTCGCG9270800.064.0244751
GGATCAT1064250.063.9121213
TCGCGGC9296100.063.9007843
GTCGCGG9300600.063.864182
GGATCTA610700.063.84113
GGATCAC462500.063.83622713
Produced by FastQC (version 0.11.2)