FastQCFastQC Report
Thu 19 Jul 2018
HY7YMBGX5_n01_dam_gr_bzip1_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHY7YMBGX5_n01_dam_gr_bzip1_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24132114
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG264200.10948066961725773No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGATC9271950.063.53724711
AGGATCC2215550.063.40302312
AGGATCT3443000.063.3353812
AGGATCA3170450.063.24846312
CGAGGAT9332300.063.20359810
GGATCCA979000.063.12941713
CGGCCGA9388250.063.0858576
GGATCTC892050.062.97007413
GGCCGAG9407350.062.9633567
GCGGCCG9407550.062.9521035
CCGAGGA9389350.062.9451839
GCCGAGG9412650.062.933668
CGCGGCC9405000.062.9010664
GGTCGCG9388350.062.8274961
TCGCGGC9409850.062.6995353
GGATCTA671750.062.69756713
GTCGCGG9414600.062.686012
GGATCAC490850.062.67415213
GGATCAT1255500.062.62918513
GGATCCC339650.062.61897713
Produced by FastQC (version 0.11.2)