FastQCFastQC Report
Thu 19 Jul 2018
HY7YMBGX5_n01_c_and_r_nlp7_h3k27ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHY7YMBGX5_n01_c_and_r_nlp7_h3k27ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59663826
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGCC40200.011.84032270
CCCGCGT33900.011.56278259
GCGGGGG23150.010.88587820
AGGGCAG84250.010.4282881
AGGGGCG42850.010.2926911
CGCACGG25950.010.11621824
CCATGCG60900.010.05725339
AGGGTTT688500.09.7409371
ATGAGTC389350.09.736381
CGCCTAG54500.09.56871238
GTCGCGA43350.09.123054564
GCCCGCG43650.09.0602158
GCGTCGC64850.09.01350121
GGCTGTC74700.08.8086492
TACCCGG55200.08.68625367
GGGCCCG80750.08.66827411
GCGATCC67450.08.61355843
TGCGTCG76100.08.50881320
AGGGACA144600.08.3514141
AGGGAAA341250.08.2982231
Produced by FastQC (version 0.11.2)