FastQCFastQC Report
Thu 19 Jul 2018
HY7YMBGX5_n01_c_and_r_nlp7_gr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHY7YMBGX5_n01_c_and_r_nlp7_gr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45815985
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGGA199550.013.17129860
GAGCTAG210050.012.529592558
AGCCATC209750.012.48112569
AGCTAGG211100.012.40094259
CGAATGA220650.011.97572640
GAAGGTC227700.011.5591014
AAGGTCT241000.010.9647615
TTATATC250550.010.63077148
CGTATGC17150.010.61190544
TAGGAAA253550.010.49032562
GTCTGAA271250.09.8187618
ACTAACG139950.09.75285110
AGAGTTA272100.09.75073621
TAACGGC139850.09.63472112
CTAACGG141800.09.55155711
TATGCCG18100.09.47534146
GCCAAGA286650.09.36500417
CCTGACG104100.09.31262814
AAAAGCC291500.09.29300266
GTATGCC20200.09.18337445
Produced by FastQC (version 0.11.2)