FastQCFastQC Report
Thu 19 Jul 2018
HY7YMBGX5_n01_c_and_r_ev_ha.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHY7YMBGX5_n01_c_and_r_ev_ha.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35356612
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCG23750.023.2862831
GTCGCGG25550.022.0553552
CGGCCGA25800.021.9771466
GCTAGGA182400.012.22230460
CGAATGA191600.011.69072340
TAACGCG165500.011.67293958
GAGCTAG189500.011.67204658
AGCTAGG191200.011.54994759
GGGCGTC135050.011.455646534
AGATTCG200250.011.32636935
AGGTCTG204850.011.24257856
GAAGGTC203750.011.1830584
ACGCGAA175350.011.15692560
CGCGAAT175550.011.08443861
AACGCGA175850.011.04559459
TCGCCCC117300.010.71112210
CGCCCCG116400.010.67364211
AGCCATC208000.010.61744269
TGACTTA212100.010.57701844
AAGGTCT221800.010.3676675
Produced by FastQC (version 0.11.2)