FastQCFastQC Report
Thu 19 Jul 2018
HY7YMBGX5_n01_c_and_r_ev_h3k27ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHY7YMBGX5_n01_c_and_r_ev_h3k27ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43548536
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCAG63700.013.9026671
CCTAGGC75750.013.21422340
CCATGCG47400.012.40479639
CCGGGCC36300.012.14844370
CGCCTAG51800.012.09450138
AGGGGCG33550.011.894041
CCCGCGT30650.011.76114259
GTCCCGA58450.010.95822648
AGGGTTT530100.010.7765421
GGGCGTG62500.010.75133610
AGGCCGT37300.010.6044141
GCGATCC57250.010.57614443
GCGTGCT60150.010.53140312
GGCGCCC16350.010.48869857
TGTCCCG59950.010.39213947
GCGACCC28700.010.1220482
GCCCGCG36700.010.10842758
GGAGTCG216550.010.08483161
TACCCGG46250.010.06452267
GTCGCGA38100.010.01278264
Produced by FastQC (version 0.11.2)