FastQCFastQC Report
Thu 19 Jul 2018
HY7YMBGX5_n01_c_and_r_ev_gr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHY7YMBGX5_n01_c_and_r_ev_gr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22920558
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGAAGGTCTGAAGAGCCAAGAGTTATTTGATGAGATTCGAATGACTTA283280.12359210452031752No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGGA133800.016.08657360
AGCTAGG135450.015.99396459
GAGCTAG134550.015.91886958
CGAATGA138700.015.39277240
AGCCATC143650.014.9595269
CGTATGC12900.014.92274646
AGGTCTG146350.014.7556316
GAAGGTC149850.014.4111144
TCGAATG150350.014.29316539
AGATTCG150150.014.19636635
TTATATC154050.014.17734248
TGACTTA153150.014.10022244
ACTTATA158350.013.72616846
GATTCGA158150.013.72118636
AAGGTCT159300.013.6441025
TATGCCG14400.013.61123548
TAGGAAA160600.013.57650562
GAGTTAT165250.013.25922422
AAGAGCC162800.013.19961613
GTATGCC14650.013.14013747
Produced by FastQC (version 0.11.2)