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        If you use plots from MultiQC in a publication or presentation, please cite:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

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        About MultiQC

        This report was generated using MultiQC, version 1.0.dev0

        You can see a YouTube video describing how to use MultiQC reports here: https://youtu.be/qPbIlO_KWN0

        For more information about MultiQC, including other videos and extensive documentation, please visit http://multiqc.info

        You can report bugs, suggest improvements and find the source code for MultiQC on GitHub: https://github.com/ewels/MultiQC

        MultiQC is published in Bioinformatics:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

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        Report generated on 2017-07-28, 13:07 based on data in: /mnt/gencore/sites/core-fastqc.bio.nyu.edu/html/HY7YJBGX2/merged


        General Statistics

        Showing 28/28 rows and 3/5 columns.
        Sample Name% Dups% GCM Seqs
        HY7YJBGX2_n01_arr1
        54.0%
        39%
        96.4
        HY7YJBGX2_n01_arr2
        38.8%
        40%
        26.5
        HY7YJBGX2_n01_fasc1
        51.9%
        40%
        48.3
        HY7YJBGX2_n01_fasc2
        51.0%
        39%
        63.4
        HY7YJBGX2_n01_nut1
        33.9%
        40%
        18.3
        HY7YJBGX2_n01_nut2
        36.4%
        39%
        17.7
        HY7YJBGX2_n01_th1
        47.1%
        39%
        53.1
        HY7YJBGX2_n01_th2
        42.9%
        38%
        27.5
        HY7YJBGX2_n01_vach1
        44.6%
        38%
        39.1
        HY7YJBGX2_n01_vach2
        42.4%
        39%
        37.4
        HY7YJBGX2_n01_vgat1
        44.3%
        39%
        39.6
        HY7YJBGX2_n01_vgat2
        42.5%
        40%
        33.8
        HY7YJBGX2_n01_vglut1
        44.8%
        39%
        54.5
        HY7YJBGX2_n01_vglut2
        43.8%
        40%
        37.3
        HY7YJBGX2_n02_arr1
        49.0%
        39%
        96.4
        HY7YJBGX2_n02_arr2
        34.8%
        40%
        26.5
        HY7YJBGX2_n02_fasc1
        48.4%
        40%
        48.3
        HY7YJBGX2_n02_fasc2
        46.0%
        40%
        63.4
        HY7YJBGX2_n02_nut1
        30.9%
        40%
        18.3
        HY7YJBGX2_n02_nut2
        32.7%
        40%
        17.7
        HY7YJBGX2_n02_th1
        43.1%
        39%
        53.1
        HY7YJBGX2_n02_th2
        40.5%
        39%
        27.5
        HY7YJBGX2_n02_vach1
        41.0%
        39%
        39.1
        HY7YJBGX2_n02_vach2
        38.6%
        39%
        37.4
        HY7YJBGX2_n02_vgat1
        40.8%
        40%
        39.6
        HY7YJBGX2_n02_vgat2
        40.0%
        40%
        33.8
        HY7YJBGX2_n02_vglut1
        40.7%
        40%
        54.5
        HY7YJBGX2_n02_vglut2
        39.8%
        41%
        37.3

        Run Statistics

        Run Statistics

        Showing 1/1 rows and 3/3 columns.
        Number of LanesTotal # of Single-End ReadsTotal # PF ReadsUndetermined
        4.0
        828721337
        629150061
        1.6%

        Demultiplexing Report

        Demultiplexing Report
        Total Read Count: Total number of PF (Passing Filter) reads in this library.
        Portion: The proportion of reads that represent the individual library in the entire Library Pool.
        Perfect: The percentage of reads in this library which have a barcode perfectly matching the barcode specified in TuboWeb. The remainder of reads have mismatches upto the max number of mismatches specified in TuboWeb (Allowed barcode mismatch option in your Library Pool).

        Showing 14/14 rows and 3/3 columns.
        LibraryTotal Read CountPortion (%)Perfect (%)
        vach1
        39071589
        6.6
        96.1
        nut2
        17688376
        3.0
        95.9
        vgat2
        33771246
        5.7
        96.3
        arr1
        96416459
        16.3
        96.2
        vglut2
        37275036
        6.3
        97.0
        vgat1
        39558481
        6.7
        95.0
        vach2
        37404819
        6.3
        95.9
        th2
        27539638
        4.6
        95.7
        arr2
        26537722
        4.5
        93.3
        fasc1
        48277523
        8.1
        96.2
        vglut1
        54470863
        9.2
        96.7
        nut1
        18264903
        3.1
        96.3
        th1
        53070884
        9.0
        95.9
        fasc2
        63383091
        10.7
        96.4

        FastQC

        FastQC is a quality control tool for high throughput sequence data, written by Simon Andrews at the Babraham Institute in Cambridge.

        Sequence Quality Histograms

        The mean quality value across each base position in the read. See the FastQC help.

        loading..

        Per Sequence Quality Scores

        The number of reads with average quality scores. Shows if a subset of reads has poor quality. See the FastQC help.

        loading..

        Per Base Sequence Content

        The proportion of each base position for which each of the four normal DNA bases has been called. See the FastQC help.

        Click a heatmap row to see a line plot for that dataset.

        rollover for sample name
        Position: -
        %T: -
        %C: -
        %A: -
        %G: -

        Per Sequence GC Content

        The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content. See the FastQC help.

        loading..

        Per Base N Content

        The percentage of base calls at each position for which an N was called. See the FastQC help.

        loading..

        Sequence Length Distribution

        All samples have sequences of a single length (75bp).


        Sequence Duplication Levels

        The relative level of duplication found for every sequence. See the FastQC help.

        loading..

        Overrepresented sequences

        The total amount of overrepresented sequences found in each library. See the FastQC help for further information.

        28 samples had less than 1% of reads made up of overrepresented sequences

        Adapter Content

        The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. See the FastQC help. Only samples with ≥ 0.1% adapter contamination are shown.

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