FastQCFastQC Report
Fri 28 Jul 2017
HY7YJBGX2_n02_vglut2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHY7YJBGX2_n02_vglut2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37275036
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2921150.7836746287783599No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACGG152000.024.67694520
TACGGGG100500.024.42732822
GTACGGG155500.024.12232421
AGAGTAC259150.022.69455718
GAGTACG169100.022.24253719
CGCAGAG264500.022.13068215
GCAGTGG296400.019.7465132
ACGCAGA299200.019.4946914
AGTGGTA304450.019.2581524
GGTATCA313700.018.9437227
GTGGTAT311200.018.918025
CAGAGTA313750.018.76718717
AACGCAG314050.018.71567313
AGCAGTG322850.018.481721
GCAGAGT319700.018.45015716
TGGTATC339700.017.5240576
TCAACGC334900.017.45697611
GAGTACT137050.017.13066320
GTATCAA363700.016.3017048
ATCAACG373400.015.97081910
Produced by FastQC (version 0.11.2)