FastQCFastQC Report
Fri 28 Jul 2017
HY7YJBGX2_n02_vglut1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHY7YJBGX2_n02_vglut1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54470863
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3624240.6653538791922573No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGGG207700.023.81975421
AGTACGG208900.023.66583420
TACGGGG134550.023.14042922
AGAGTAC354500.022.25051718
GAGTACG223450.022.17107219
CGCAGAG360650.021.87070815
ACGCAGA418600.018.90031414
AGTGGTA428400.018.6589784
GCAGTGG431450.018.3192182
GGTATCA436600.018.3010757
CAGAGTA443950.018.10124817
GCAGAGT446350.017.81082716
TCCGATC198350.017.7781246
GTGGTAT450150.017.7726765
AACGCAG449250.017.72583413
AGCAGTG462800.017.3546811
TCAACGC464650.017.14511911
CCGATCT211500.016.8359187
GAGTACT202100.016.56395320
TGGTATC484600.016.5166266
Produced by FastQC (version 0.11.2)