FastQCFastQC Report
Fri 28 Jul 2017
HY7YJBGX2_n02_vgat2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHY7YJBGX2_n02_vgat2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33771246
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2182730.646327944192524No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGG90650.025.48481622
AGTACGG137800.025.36922620
GTACGGG137600.025.23149921
CGCAGAG232700.023.18560815
AGAGTAC233750.023.02287318
GAGTACG159650.022.24266419
AGTGGTA266350.020.473374
ACGCAGA264600.020.41575614
GCAGTGG266800.020.322172
GTGGTAT270850.019.9805895
AACGCAG280400.019.26522613
CAGAGTA283700.019.16380917
GGTATCA288650.018.9279777
AGCAGTG292950.018.8499831
GCAGAGT289350.018.81346516
TCAACGC302500.017.93683411
TGGTATC306100.017.8375196
GTATCAA320300.017.1333248
GAGTACT121150.017.04597920
ATCAACG339500.016.25608810
Produced by FastQC (version 0.11.2)