FastQCFastQC Report
Fri 28 Jul 2017
HY7YJBGX2_n02_vgat1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHY7YJBGX2_n02_vgat1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39558481
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2839940.7179092645139736No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGGG147050.024.00788721
TACGGGG95950.023.32153722
AGTACGG156500.022.73378620
AGAGTAC264350.021.0875518
CGCAGAG265350.021.0336915
GAGTACG180900.019.83876819
GAGTACT143300.019.2464320
GCAGTGG304250.018.703622
AGTGGTA311350.018.3884124
ACGCAGA306350.018.37584114
GTGGTAT317900.017.966445
CAGAGTA320100.017.63029117
GGTATCA324950.017.566447
GCAGAGT319150.017.5639516
AGCAGTG336850.017.1503731
TCAACGC333250.017.04686511
AACGCAG331700.017.02346213
TGGTATC354550.015.9441296
GTATCAA364700.015.6710278
CAACGCA367650.015.61158812
Produced by FastQC (version 0.11.2)