FastQCFastQC Report
Fri 28 Jul 2017
HY7YJBGX2_n02_vach2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHY7YJBGX2_n02_vach2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37404819
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2154360.5759578732355315No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA405720.10846730738090192No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGGG153100.025.33350221
AGTACGG159050.024.3633620
TACGGGG106400.023.72120322
AGAGTAC268000.022.72984118
CGCAGAG266800.022.7026815
GAGTACG175050.022.2743519
AGTGGTA301950.020.7649464
GCAGTGG302050.020.6436482
ACGCAGA305600.019.7972614
GTGGTAT315400.019.7917985
GGTATCA320200.019.4633837
CAGAGTA318000.019.2861917
AGCAGTG331050.018.9193441
GCAGAGT323100.018.8750916
AACGCAG324450.018.68949913
TCAACGC326500.018.6876711
TGGTATC339700.018.396636
GAGTACT140650.017.67258520
GTATCAA360350.017.2091318
CAACGCA361250.016.85213312
Produced by FastQC (version 0.11.2)