FastQCFastQC Report
Fri 28 Jul 2017
HY7YJBGX2_n02_vach1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHY7YJBGX2_n02_vach1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39071589
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1823160.46662038751482565No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA690840.17681389922483062No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGGG150100.025.54132721
TACGGGG106550.024.56128122
AGTACGG162050.023.95492620
AGAGTAC268550.022.5934718
CGCAGAG273950.022.12255315
GAGTACG184650.021.07908419
AGTGGTA310000.019.8700684
GCAGTGG314100.019.6105522
ACGCAGA315700.019.1965714
TCAACGC324050.018.9673411
GCAGAGT322100.018.92284616
GTGGTAT325900.018.8795435
CAGAGTA325850.018.80029317
GGTATCA336550.018.5385157
AGCAGTG339100.018.3993431
AACGCAG336650.018.25799813
GAGTACT147450.017.86298620
TGGTATC353000.017.547626
CAACGCA359650.017.10917912
GTATCAA366750.016.9182578
Produced by FastQC (version 0.11.2)