FastQCFastQC Report
Fri 28 Jul 2017
HY7YJBGX2_n02_th2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHY7YJBGX2_n02_th2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27539638
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2006930.7287423313262142No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACGG98400.024.80715620
GTACGGG98850.024.66015221
TACGGGG68150.024.33756822
CGCAGAG181550.022.10449215
AGAGTAC186400.021.43714118
ACGCAGA207900.019.23620814
AGTGGTA208800.019.1840864
GAGTACG130200.019.11909919
GCAGTGG213100.018.894112
CAGAGTA213350.018.79384417
GAGTACT105400.018.67817120
AGCAGTG218400.018.6100671
GGTATCA218250.018.5277147
TCAACGC217800.018.2977211
GTGGTAT219050.018.2548665
AACGCAG219850.018.22181513
GCAGAGT226000.017.863916
TGGTATC237600.017.0765556
CAACGCA244000.016.5309812
GTATCAA247800.016.221188
Produced by FastQC (version 0.11.2)