FastQCFastQC Report
Fri 28 Jul 2017
HY7YJBGX2_n02_th1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHY7YJBGX2_n02_th1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53070884
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3280820.6181958453904781No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGGG199650.024.86655421
AGTACGG205250.024.30469520
TACGGGG136950.024.0946522
CGCAGAG358100.021.95911215
AGAGTAC358750.021.90020818
GAGTACG256500.019.82490519
GCAGTGG409550.019.3991572
ACGCAGA407000.019.35422314
AGTGGTA414300.019.2103674
AGCAGTG417400.019.2083681
CAGAGTA416550.018.9689517
GAGTACT196950.018.7472820
TCAACGC427150.018.448211
GGTATCA431500.018.4450767
AACGCAG436650.018.12673213
GTGGTAT441000.018.0239455
GCAGAGT442950.017.98633616
TGGTATC469400.016.9335586
CAACGCA484150.016.3976812
GTATCAA489400.016.2208128
Produced by FastQC (version 0.11.2)