FastQCFastQC Report
Fri 28 Jul 2017
HY7YJBGX2_n02_nut2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHY7YJBGX2_n02_nut2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17688376
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1280770.7240743864784421No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA187290.10588309520331318No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA83250.055.68507847
CGTGTAG114200.055.0026616
GTAGGGA111100.054.3672619
GCCGTAT101500.052.36509748
GAGCGTC164300.052.2008310
TGTAGGG119600.051.85731518
AGCGTCG167750.051.00424211
CGTCGTG170550.050.22950713
AGAGCGT172350.050.0625469
GCGTCGT170500.049.87902512
GTGTAGG126200.049.2541417
GTCGTGT166100.049.00191514
TAGGGAA123900.048.91785420
GTCGCCG108800.048.2583245
TCGTGTA162750.047.4911215
AAGAGCG185150.047.066368
GGTCGCC117750.046.8490744
TCGCCGT99950.046.07446746
GAGTGTA117700.045.86282328
CCGTATC90200.045.3099749
Produced by FastQC (version 0.11.2)