FastQCFastQC Report
Fri 28 Jul 2017
HY7YJBGX2_n02_nut1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHY7YJBGX2_n02_nut1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18264903
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1257650.6885610068665571No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA248650.13613540679630218No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACGG75550.026.10254120
GTACGGG75900.025.89217621
TACGGGG52300.025.448622
GAGTACG80000.024.77987119
AGAGTAC132800.024.76628118
CGCAGAG136500.023.81687715
AGTGGTA154050.021.3915624
ACGCAGA154300.021.13599214
CAGAGTA157500.020.9479117
GGTATCA161350.020.381297
GCAGTGG162000.020.3194562
GTGGTAT161900.020.2692455
AACGCAG161700.020.10460513
GCAGAGT164650.019.82874316
AGCAGTG170750.019.4808081
TCAACGC167550.019.3399211
TGGTATC172450.019.1292276
CAACGCA183700.017.94044112
GTATCAA185050.017.9203038
GAGTACT74300.017.58618419
Produced by FastQC (version 0.11.2)