FastQCFastQC Report
Fri 28 Jul 2017
HY7YJBGX2_n02_fasc2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHY7YJBGX2_n02_fasc2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63383091
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4461870.7039527308631888No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1252520.19761106317771723No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGGG347500.027.4224321
TACGGGG233250.027.2591122
AGTACGG354650.027.1604420
GAGTACG364200.026.54288519
AGAGTAC622150.025.75560618
CGCAGAG625950.025.68155915
GCAGTGG668250.024.098042
AGCAGTG680400.023.7801251
AGTGGTA681500.023.7517074
GGTATCA694300.023.3043547
ACGCAGA692700.023.1464914
GTGGTAT702650.023.0074275
CAGAGTA710600.022.73904617
AACGCAG711450.022.5267113
GCAGAGT718450.022.41381516
TCAACGC721850.022.1581811
TGGTATC752750.021.4305766
GTATCAA776500.020.988798
CAACGCA791050.020.37707512
ATCAACG814800.019.87147910
Produced by FastQC (version 0.11.2)