FastQCFastQC Report
Fri 28 Jul 2017
HY7YJBGX2_n02_fasc1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHY7YJBGX2_n02_fasc1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48277523
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1783860.36950114445598214No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGGG185400.024.11912221
AGTACGG185950.023.97289820
TACGGGG129450.023.8127722
AGAGTAC323500.021.86818318
CGCAGAG326600.021.52303515
GAGTACG213050.021.21468719
GCAGTGG361250.019.8087352
AGTGGTA367300.019.6235894
GTGGTAT373750.019.0634485
ACGCAGA371850.018.97759814
AACGCAG392600.018.07120913
CAGAGTA397400.017.92311317
GAGTACT180850.017.63361720
GGTATCA411900.017.45727
AGCAGTG417900.017.3225231
GCAGAGT414200.017.17112516
TGGTATC431550.016.5582836
TCAACGC439350.016.22599411
GTATCAA472250.015.2411998
ATCAACG486650.014.86129310
Produced by FastQC (version 0.11.2)