FastQCFastQC Report
Fri 28 Jul 2017
HY7YJBGX2_n02_arr2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHY7YJBGX2_n02_arr2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26537722
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1842550.6943135511028414No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACGG113600.023.57989720
TACGGGG77750.023.41517822
GTACGGG116200.023.082721
CGCAGAG191900.021.91628315
AGAGTAC190800.021.84430118
GAGTACG125850.021.53113719
ACGCAGA215200.019.46313514
GCAGTGG219600.018.8980642
AGTGGTA221900.018.8733354
AGCAGTG223600.018.5913161
GTGGTAT226600.018.4973665
AACGCAG230800.018.31190313
GCAGAGT231600.018.2342816
GGTATCA234650.018.024587
CAGAGTA234350.017.99088717
GAGTACT102200.017.81075320
TCAACGC240650.017.3754711
TGGTATC250900.016.7747546
GTATCAA266650.015.9135638
CAACGCA270900.015.61368212
Produced by FastQC (version 0.11.2)