FastQCFastQC Report
Fri 28 Jul 2017
HY7YJBGX2_n02_arr1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHY7YJBGX2_n02_arr1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96416459
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7475500.7753344270815836No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGGG322450.022.43158721
TACGGGG224650.022.423622
AGTACGG334800.021.80921720
CGCAGAG585300.020.2377315
AGAGTAC585400.020.23436718
GAGTACG415600.017.71011719
ACGCAGA673250.017.54239714
GCAGTGG675850.017.4217532
AGTGGTA685350.017.2407044
GTGGTAT698000.016.9430795
TCCGATC323650.016.8910666
CAGAGTA717700.016.64857517
GAGTACT375800.016.46672420
AACGCAG720050.016.45011313
CCGATCT337900.016.3217417
TCAACGC723850.016.27734811
AGCAGTG743650.016.2002961
GCAGAGT737300.016.19668816
GGTATCA767050.015.8090887
TGGTATC771800.015.4525746
Produced by FastQC (version 0.11.2)